CellAging is a tool designed to automatically extract information on the segregation of aggregates to the cell poles and their partitioning in division and on cellular vitality from temporal images of E. coli cells, obtained by parallel brightfield and fluorescence microscopy. CellAging performs cell segmentation from brightfield images, alignment of brightfield and fluorescence images, lineage construction and pole age determination, and computation of aging-related features. An example of its use analyzing tsr-venus protein distributions from live cell images across a few generations can also be downloaded below.

Reference: A. Häkkinen, A.-B. Muthukrishnan, A. Mora, J.M. Fonseca, and A.S. Ribeiro (2013) CellAging: A tool to study segregation and partitioning in division in cell lineages of Escherichia coli. Bioinformatics 29 (13): 1708-1709. doi: 10.1093/bioinformatics/btt194