ZebIAT is a tool for registering zebrafish images acquired by
fluorescence microscope as well as differential interference
microscope. The tool offers automatic and semi-automatic methods to
analyze images and thus allow, e.g. quantitative comparisons between
images. The tool uses a thin plate splines registration method.
Reference: T. Annila, E. Lihavainen, I.J. Marques, D.R. Williams, O. Yli-Harja and A.S. Ribeiro. 2013. ZebIAT, an image analysis tool for registering zebrafish embryos and quantifying cancer metastasis. BMC Bioinformatics 14 (Sup. 10), S5, in press.
Zebratool requires also some addition files in order to work. For spot detection we used wavelet segmentation algorithm based on article "Ruusuvuori et al.: Evaluation of methods for detection of fluorescence labeled subcellular objects in microscope images". For interpolation we used "inpaint" technique which can be downloaded from Mathworks:"Inpaint"-method
We also provide a set of fluorescence microscope images of zebrafish for testing. The guide for using the tool is also provided.Example images